Anti-Human PERP Recombinant Antibody (KM3411) (CAT#: TAB-1017CL)

Figure 1 Reactivity of KM3411 in FMAT.

In the graph, the ordinate shows the integral value of fluorescence intensity and cell numbers.

Figure 2 Reactivity of KM3411 in flow cytometry.

The ordinate and the abscissa in each drawings show cell numbers and fluorescence intensity, respectively.

Figure 3 Reactivity of KM3411 in flow cytometry.

The ordinate shows the ratio of a mean fluorescence intensity of KM3411 when a mean intensity of KM511 which is a negative control is defined as 1. Upper numerals in the graph show the values when ratio of mean fluorescence intensity was 15 or more in particular, Table in the drawing shows the cell lines used.

Figure 1 Anti-Human PERP Antibody (TAB-1017CL) in SDS-PAGE

SDS-PAGE analysis of TAB-1017CL in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Anti-Human PERP Antibody (TAB-1017CL) in HPLC

HPLC analysis of TAB-1017CL shows the purity is>95%.

Figure 3 Anti-Human PERP Antibody (TAB-1017CL) in Flow cytometry

Flow cytometric analysis of human PERP expression on HEK293 cells. Cells were stained with purified Anti-Human PERP Recombinant Antibody (TAB-1017CL) in 25 ug/ml. The secondary antibody is FITC-Anti-mouse IgG.
(1) 293-NC1: HEK293-negtive control, incubated with TAB-1017CL, secondary antibody FITC-Anti-mouse IgG;
(2) Model-FITC: PERP-expressed HEK293, incubated with TAB-1017CL, secondary antibody FITC-Anti-mouse IgG;